4-week-old and 42-week-old C57BL/6 male mice were fed Antibacterial Compound Library Data Sheet control diet (CD) or control diet mce In stock supplemented with quercetin, -tocotrienol or dexamethasone for 4 week. CD = control diet; Quer = quercetin; Trie = -tocotrienol; Dexa = dexamethasone.Qureshi et al. Lipids in Health PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26278330 and Disease 2011, 10:239 http://www.lipidworld.com/content/10/1/Page 13 of8-week-old C57BL/6 mice (LPS 100 ng).46-week-old C57BL/6 mice (LPS 100 ng).Antifungal Compound Library web Figure 6 Effects of dietary supplementation with various compounds on the secretion of TNF-a by thioglycolate-elicited peritoneal macrophages stimulated with LPS. 4-week-old and 42-week-old C57BL/6 male mice were fed control diet or control diet supplemented with quercetin, -tocotrienol or dexamethasone for 4 week. After this, thioglycolate-elicited peritoneal macrophages were collected, adhered to the bottom of 12 well plates (1?07 cells/well in 1.0 mL medium) for 4 h, then washed with medium three times. Then cells were treated with LPS (100 ng/well), and incubated at 37 in 5 CO2 for 4 h [9]. Supernatants were used to carry out TNF-a assays by ELISA. Data are means ?SD; n = 3 mice per group, with triplicate analysis of each sample. Values in columns with different symbols differ at P < 0.05. Data represent means ?SD; n = 3 mice per group, with triplicate analysis of each sample. Values in columns with different symbols differ at P < 0.05. Treatments 1 - 16 correspond to: 1 - 4 = 8-week-old mice or 5 - 8 = 46-week-old mice treated with 10 ng/well LPS; 9 - 12 = 8-week-old mice or 13 - 16 = 46-week-old mice treated with 10 ng LPS plus + 50 U/well IFN-g. CD = control diet; Quer = quercetin; Trie = -tocotrienol; Dexa = dexamethasone.Qureshi et al. Lipids in Health PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26278330 and Disease 2011, 10:239 http://www.lipidworld.com/content/10/1/Page 13 of8-week-old C57BL/6 mice (LPS 100 ng).46-week-old C57BL/6 mice (LPS 100 ng).Figure 6 Effects of dietary supplementation with various compounds on the secretion of TNF-a by thioglycolate-elicited peritoneal macrophages stimulated with LPS. 4-week-old and 42-week-old C57BL/6 male mice were fed control diet or control diet supplemented with quercetin, -tocotrienol or dexamethasone for 4 week. After this, thioglycolate-elicited peritoneal macrophages were collected, adhered to the bottom of 12 well plates (1?07 cells/well in 1.0 mL medium) for 4 h, then washed with medium three times. Then cells were treated with LPS (100 ng/well), and incubated at 37 in 5 CO2 for 4 h [9]. Supernatants were used to carry out TNF-a assays by ELISA. Data are means ?SD; n = 3 mice per group, with triplicate analysis of each sample. Values in columns with different symbols differ at P < 0.05. The treatments 1 - 8 correspond to: 1 - 4 = 8-week-old mice or 5 - 8 = 46-week-old mice treated with 100 ng LPS. CD = control diet; Quer = quercetin; Trie = tocotrienol; Dexa = dexamethasone.Qureshi et al.